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Product Description
Human MEC (Mucosae Associated Epithelia Chemokine) ELISA Kit | ELK4889 | ELK Biotechnology
Alternative Names: CCL28; SCYA28; Small Inducible Cytokine Subfamily A (Cys-Cys) Member 28; Chemokine C-C-Motif Ligand 28; Small-inducible cytokine A28
Species: Human
Assay Type: Sandwich
Sensitivity: 0.059 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Assay Lenght: 3.5h
Research Area: Cytokine;Tumor immunity;Infection immunity;Dermatology
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mucosae Associated Epithelia Chemokine (MEC) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mucosae Associated Epithelia Chemokine (MEC) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mucosae Associated Epithelia Chemokine (MEC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mucosae Associated Epithelia Chemokine (MEC) in the samples is then determined by comparing the OD of the samples to the standard curve.
