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Product Description
Human MGAT5 (Mannosyl Alpha-1, 6-Glycoprotein Beta-1, 6-N-Acetylglucosaminyltransferase) ELISA Kit | ELK5165 | ELK Biotechnology
Alternative Names: GNT-V; GGNT5; Mannoside acetylglucosaminyltransferase 5; N-acetylglucosaminyl-transferase V; Alpha-1, 6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A
Species: Human
Assay Type: Sandwich
Sensitivity: 0.067 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: Serum, plasma, tissue homogenates and other biological fluids.
Assay Lenght: 3.5h
Research Area: Enzyme & Kinase
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mannosyl Alpha-1, 6-Glycoprotein Beta-1, 6-N-Acetylglucosaminyltransferase (MGAT5) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mannosyl Alpha-1, 6-Glycoprotein Beta-1, 6-N-Acetylglucosaminyltransferase (MGAT5) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mannosyl Alpha-1, 6-Glycoprotein Beta-1, 6-N-Acetylglucosaminyltransferase (MGAT5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mannosyl Alpha-1, 6-Glycoprotein Beta-1, 6-N-Acetylglucosaminyltransferase (MGAT5) in the samples is then determined by comparing the OD of the samples to the standard curve.
