Euro
USD
British Pound
NULL
Product Description
Human MSH6 (mutS homolog 6) ELISA Kit | ELK9128 | ELK Biotechnology
Alternative Names: DNA mismatch repair protein Msh6; G/T mismatch binding protein; GTBP; MutS homolog 6 (E. coli) ; mutS homolog 6
Species: Human
Assay Type: Sandwich
Sensitivity: 6.8 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, saliva, cell culture supernates and other biological fluids
Assay Lenght: 3.5h
Research Area: Epigenetics and Nuclear Signaling
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to mutS homolog 6 (MSH6) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to mutS homolog 6 (MSH6) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain mutS homolog 6 (MSH6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of mutS homolog 6 (MSH6) in the samples is then determined by comparing the OD of the samples to the standard curve.
