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Product Description
Human mTOR (MammalianTarget of Rapamycin) ELISA Kit | ELK9591 | ELK Biotechnology
Alternative Names: FRAP1, RAFT1, RAPT1, Mechanistic target of rapamycin, FK506 binding protein 12-rapamycin associated protein 1
Species: Human
Assay Type: Sandwich
Sensitivity: 0.10 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Lenght: 3.5h
Research Area: Cell proliferation
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to MammalianTarget of Rapamycin (mTOR) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to MammalianTarget of Rapamycin (mTOR) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain MammalianTarget of Rapamycin (mTOR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of MammalianTarget of Rapamycin (mTOR) in the samples is then determined by comparing the OD of the samples to the standard curve.
