Product Description
Human MYOZ2 (Myozenin 2) ELISA Kit | ELK4171 | ELK Biotechnology
Alternative Names: CS-1; C4orf5; Calsarcin; Calcineurin Associated Sarcomeric Protein; FATZ-related protein 2
Species: Human
Assay Type: Sandwich
Sensitivity: 0.061 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: Tissue homogenates and other biological fluids.
Assay Lenght: 3.5h
Research Area: Metabolic pathway
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Myozenin 2 (MYOZ2) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Myozenin 2 (MYOZ2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Myozenin 2 (MYOZ2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Myozenin 2 (MYOZ2) in the samples is then determined by comparing the OD of the samples to the standard curve.