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Product Description
Human NCF2 (Neutrophil Cytosolic Factor 2) ELISA Kit | ELK9550 | ELK Biotechnology
Alternative Names: NOXA2; p67phox; 65kDa, Chronic Granulomatous Disease, Autosomal 2; NADPH Oxidase Activator 2; 67 kDa neutrophil oxidase factor; Neutrophil NADPH oxidase factor 2
Species: Human
Assay Type: Sandwich
Sensitivity: 0.113 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: Tissue homogenates, cell lysates and other biological fluids
Assay Lenght: 3.5h
Research Area: Infection immunity
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neutrophil Cytosolic Factor 2 (NCF2) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Neutrophil Cytosolic Factor 2 (NCF2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Neutrophil Cytosolic Factor 2 (NCF2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Neutrophil Cytosolic Factor 2 (NCF2) in the samples is then determined by comparing the OD of the samples to the standard curve.
