Euro
USD
British Pound
NULL
Product Description
Human NFKB-p65 (Nuclear Factor Kappa B p65) ELISA Kit | ELK9589 | ELK Biotechnology
Alternative Names: NFKB3, p65, RelA
Species: Human
Assay Type: Sandwich
Sensitivity: 0.10 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Lenght: 3.5h
Research Area: Signal transduction
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nuclear Factor Kappa B p65 (NFKB-p65) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Nuclear Factor Kappa B p65 (NFKB-p65) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Nuclear Factor Kappa B p65 (NFKB-p65), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Nuclear Factor Kappa B p65 (NFKB-p65) in the samples is then determined by comparing the OD of the samples to the standard curve.
