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Product Description
Human NPPB (Natriuretic peptide precursor B) ELISA Kit | ELK9382 | ELK Biotechnology
Alternative Names: ANFB_HUMAN; BNP; BNP (1-32) ; BNP (5-29) ; BNP-32; Brain type natriuretic peptide; Gamma brain natriuretic peptide; Gamma-brain natriuretic peptide; Natriuretic peptide precursor B; nppb; NT proBNP
Species: Human
Assay Type: Sandwich
Sensitivity: 0.55 ng/mL
Standard: 100 ng/mL
Detection Range: 1.57-100 ng/mL
Sample Type: Serum, plasma, tissue homogenates and other biological fluids.
Assay Lenght: 3.5h
Research Area: Neuroscience; Cardiovascular
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Natriuretic peptide precursor B (NPPB) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Natriuretic peptide precursor B (NPPB) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Natriuretic peptide precursor B (NPPB), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Natriuretic peptide precursor B (NPPB) in the samples is then determined by comparing the OD of the samples to the standard curve.
