Product Description
Human NRXN3 (Neurexin3) ELISA Kit | ELK8785 | ELK Biotechnology
Alternative Names: NRXN3; Neurexin-3; Neurexin III-alpha; Neurexin-3-alpha; C14orf60; KIAA0743
Species: Human
Assay Type: Sandwich
Sensitivity: 12.7 pg/mL
Standard: 2000 pg/mL
Detection Range: 31.25-2000 pg/mL
Sample Type: serum, plasma and other biological fluids
Assay Lenght: 3.5h
Research Area: Autistic Disorder
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neurexin3 (NRXN3) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Neurexin3 (NRXN3) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Neurexin3 (NRXN3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Neurexin3 (NRXN3) in the samples is then determined by comparing the OD of the samples to the standard curve.