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Product Description
Human OLIG2 (Oligodendrocyte Lineage Transcription Factor 2) ELISA Kit | ELK4880 | ELK Biotechnology
Alternative Names: BHLHB1; OLIGO2; PRKCBP2; RACK17; Basic Domain, Helix-Loop-Helix Protein, Class B 1; Class B basic helix-loop-helix protein 1; Class E basic helix-loop-helix protein 19
Species: Human
Assay Type: Sandwich
Sensitivity: 5.7 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: Tissue homogenates, cell lysates and other biological fluids.
Assay Lenght: 3.5h
Research Area: Neuro science
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Oligodendrocyte Lineage Transcription Factor 2 (OLIG2) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Oligodendrocyte Lineage Transcription Factor 2 (OLIG2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Oligodendrocyte Lineage Transcription Factor 2 (OLIG2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Oligodendrocyte Lineage Transcription Factor 2 (OLIG2) in the samples is then determined by comparing the OD of the samples to the standard curve.
