Euro
USD
British Pound
NULL
Product Description
Human PDE10A (Phosphodiesterase 10A) ELISA Kit | ELK4132 | ELK Biotechnology
Alternative Names: cAMP and cAMP-inhibited cGMP 3', 5'-cyclic phosphodiesterase 10A
Species: Human
Assay Type: Sandwich
Sensitivity: 0.104 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: Tissue homogenates and other biological fluids.
Assay Lenght: 3.5h
Research Area: Enzyme & Kinase
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phosphodiesterase 10A (PDE10A) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Phosphodiesterase 10A (PDE10A) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phosphodiesterase 10A (PDE10A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Phosphodiesterase 10A (PDE10A) in the samples is then determined by comparing the OD of the samples to the standard curve.
