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Product Description
Human PIK3C3 (Phosphoinositide-3-Kinase Class 3) ELISA Kit | ELK5138 | ELK Biotechnology
Alternative Names: Vps34; Phosphatidylinositol 3-kinase p100 subunit; Phosphatidylinositol 3-kinase catalytic subunit type 3
Species: Human
Assay Type: Sandwich
Sensitivity: 0.59 ng/mL
Standard: 100 ng/mL
Detection Range: 1.57-100 ng/mL
Sample Type: Tissue homogenates, cell lysates and other biological fluids
Assay Lenght: 3.5h
Research Area: Enzyme & Kinase
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phosphoinositide-3-Kinase Class 3 (PIK3C3) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Phosphoinositide-3-Kinase Class 3 (PIK3C3) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phosphoinositide-3-Kinase Class 3 (PIK3C3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Phosphoinositide-3-Kinase Class 3 (PIK3C3) in the samples is then determined by comparing the OD of the samples to the standard curve.
