Euro
USD
British Pound
NULL
Product Description
Human PTK6 (Protein Tyrosine Kinase 6) ELISA Kit | ELK4921 | ELK Biotechnology
Alternative Names: BRK; Breast tumor kinase; Tyrosine-protein kinase BRK
Species: Human
Assay Type: Sandwich
Sensitivity: 0.58 ng/mL
Standard: 100 ng/mL
Detection Range: 1.57-100 ng/mL
Sample Type: Tissue homogenates, cell lysates and other biological fluids
Assay Lenght: 3.5h
Research Area: Enzyme & Kinase
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Tyrosine Kinase 6 (PTK6) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Protein Tyrosine Kinase 6 (PTK6) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Tyrosine Kinase 6 (PTK6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protein Tyrosine Kinase 6 (PTK6) in the samples is then determined by comparing the OD of the samples to the standard curve.
