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Product Description
Human RBM8 (RNA Binding Motif Protein 8) ELISA Kit | ELK5333 | ELK Biotechnology
Alternative Names: RBM8A; BOV-1A; BOV-1B; BOV-1C; RBM8B; Y14; ZNRP; ZRNP1; Binder of OVCA1-1; RNA-binding protein Y14; Ribonucleoprotein RBM8A
Species: Human
Assay Type: Sandwich
Sensitivity: 0.063 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: Tissue homogenates, cell lysates and other biological fluids
Assay Lenght: 3.5h
Research Area: Signal transduction
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to RNA Binding Motif Protein 8 (RBM8) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to RNA Binding Motif Protein 8 (RBM8) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain RNA Binding Motif Protein 8 (RBM8), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of RNA Binding Motif Protein 8 (RBM8) in the samples is then determined by comparing the OD of the samples to the standard curve.
