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Product Description
Human sCD130 (Soluble CD130) ELISA Kit | ELK9349 | ELK Biotechnology
Alternative Names: CDw130; CD130; IL6ST; GP130-RAPS; IR6RB; IL6R-Beta; IR6RB; IR6-RB; Interleukin 6 Signal Transducer; Oncostatin M Receptor; Interleukin-6 Receptor Subunit Beta
Species: Human
Assay Type: Sandwich
Sensitivity: 48 pg/mL
Standard: 8000 pg/mL
Detection Range: 125-8000 pg/mL
Sample Type: Serum, plasma and other biological fluids.
Assay Lenght: 3.5h
Research Area: Signal transduction;Infection immunity
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Soluble CD130 (sCD130) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Soluble CD130 (sCD130) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Soluble CD130 (sCD130), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Soluble CD130 (sCD130) in the samples is then determined by comparing the OD of the samples to the standard curve.
