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Product Description
Human sIL30 (Soluble Interleukin 30) ELISA Kit | ELK9351 | ELK Biotechnology
Alternative Names: IL-27;p28; IL30; Interleukin 30; Interleukin-27 subunit alpha
Species: Human
Assay Type: Sandwich
Sensitivity: 2.9 pg/mL
Standard: 500 pg/mL
Detection Range: 7.82-500 pg/mL
Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Assay Lenght: 3.5h
Research Area: Cytokine;Tumor immunity;Infection immunity
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Soluble Interleukin 30 (sIL30) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Soluble Interleukin 30 (sIL30) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Soluble Interleukin 30 (sIL30), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Soluble Interleukin 30 (sIL30) in the samples is then determined by comparing the OD of the samples to the standard curve.
