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Product Description
Human SOD4 (Superoxide Dismutase, Copper Chaperone) ELISA Kit | ELK4351 | ELK Biotechnology
Alternative Names: CCS; Copper Chaperone For Superoxide Dismutase
Species: Human
Assay Type: Sandwich
Sensitivity: 0.115 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: Tissue homogenates, cell lysates and other biological fluids
Assay Lenght: 3.5h
Research Area: Enzyme & Kinase;Metabolic pathway;Developmental science
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Superoxide Dismutase, Copper Chaperone (SOD4) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Superoxide Dismutase, Copper Chaperone (SOD4) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Superoxide Dismutase, Copper Chaperone (SOD4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Superoxide Dismutase, Copper Chaperone (SOD4) in the samples is then determined by comparing the OD of the samples to the standard curve.
