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Product Description
Human SP140 (Sp140 Nuclear Body Protein) ELISA Kit | ELK7335 | ELK Biotechnology
Alternative Names: LYSP100-B; LYSP100-A; Lymphoid-restricted homolog of Sp100; Nuclear autoantigen Sp-140; Speckled 140 kDa
Species: Human
Assay Type: Sandwich
Sensitivity: 0.053 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: Tissue homogenates, cell lysates and other biological fluids.
Assay Lenght: 3.5h
Research Area: Signal transduction
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sp140 Nuclear Body Protein (SP140) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Sp140 Nuclear Body Protein (SP140) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sp140 Nuclear Body Protein (SP140), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sp140 Nuclear Body Protein (SP140) in the samples is then determined by comparing the OD of the samples to the standard curve.
