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Product Description
Human SPTAN1 (Alpha-Fodrin) ELISA Kit | ELK9098 | ELK Biotechnology
Alternative Names: SPTAN1; NEAS; (ALPHA) II-SPECTRIN; Spectrin Alpha Non-Erythrocytic 1; Spectrin Alpha Chain, Brain; Spectrin, non-erythroid alpha subunit
Species: Human
Assay Type: Sandwich
Sensitivity: 6.8 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: Tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Assay Lenght: 3.5h
Research Area: Signal transduction;Apoptosis
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alpha-Fodrin (SPTAN1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Alpha-Fodrin (SPTAN1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Alpha-Fodrin (SPTAN1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Alpha-Fodrin (SPTAN1) in the samples is then determined by comparing the OD of the samples to the standard curve.
