Product Description
Human STAB1 (Stabilin-1) ELISA Kit | ELK9231 | ELK Biotechnology
Alternative Names: STAB1 ; Stabilin-1 ; Fasciclin ; EGF-like ; laminin-type EGF-like and link domain-containing scavenger receptor 1 ; FEEL-1 ; MS-1 antigen ; FEEL1 ; KIAA0246 ; FEX1 ; FELE-1 ; STAB-1 ; CLEVER-1 ; common lymphatic endothelial and vascular endothelial receptor-1
Species: Human
Assay Type: Sandwich
Sensitivity: 0.056 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates and other biological fluids
Assay Lenght: 3.5h
Research Area: Signal Transduction; Epigenetics and Nuclear Signaling
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Stabilin-1 (STAB1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Stabilin-1 (STAB1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Stabilin-1 (STAB1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Stabilin-1 (STAB1) in the samples is then determined by comparing the OD of the samples to the standard curve.