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Product Description
Human TRAF3 (TNF Receptor Associated Factor 3) ELISA Kit | ELK4372 | ELK Biotechnology
Alternative Names: LAP1; CAP-1; CD40bp; CRAF1; CD40 receptor-associated factor 1; CD40-binding protein; LMP1-associated protein 1
Species: Human
Assay Type: Sandwich
Sensitivity: 6.3 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: Serum, plasma, tissue homogenates, cell lysates and other biological fluids.
Assay Lenght: 3.5h
Research Area: Tumor immunity
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to TNF Receptor Associated Factor 3 (TRAF3) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to TNF Receptor Associated Factor 3 (TRAF3) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TNF Receptor Associated Factor 3 (TRAF3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of TNF Receptor Associated Factor 3 (TRAF3) in the samples is then determined by comparing the OD of the samples to the standard curve.
