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Product Description
Human UCN2 (Urocortin 2) ELISA Kit | ELK5298 | ELK Biotechnology
Alternative Names: URP; SRP; UCN-II; UCNII; Stresscopin-Related Peptide; Urocortin-related peptide
Species: Human
Assay Type: Sandwich
Sensitivity: 3.1 pg/mL
Standard: 500 pg/mL
Detection Range: 7.82-500 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Lenght: 3.5h
Research Area: Endocrinology
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Urocortin 2 (UCN2) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Urocortin 2 (UCN2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Urocortin 2 (UCN2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Urocortin 2 (UCN2) in the samples is then determined by comparing the OD of the samples to the standard curve.
