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Product Description
Human ZBED3 (Zinc Finger, BED Type Containing Protein 3) ELISA Kit | ELK6719 | ELK Biotechnology
Alternative Names: Axin-interacting protein
Species: Human
Assay Type: Sandwich
Sensitivity: 0.128 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: Tissue homogenates, cell lysates and other biological fluids.
Assay Lenght: 3.5h
Research Area: Signal transduction
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Zinc Finger, BED Type Containing Protein 3 (ZBED3) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Zinc Finger, BED Type Containing Protein 3 (ZBED3) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Zinc Finger, BED Type Containing Protein 3 (ZBED3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Zinc Finger, BED Type Containing Protein 3 (ZBED3) in the samples is then determined by comparing the OD of the samples to the standard curve.
