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Product Description
Human ADAM8 (A Disintegrin And Metalloprotease 8) ELISA Kit | ELK1695 | ELK Biotechnology
Alternative Names: CD156-A; MS2; CD156a; Disintegrin and metalloproteinase domain-containing protein 8
Species: Human
Assay Type: Sandwich
Sensitivity: 2.37 pg/mL
Standard: 400 pg/mL
Detection Range: 6.25-400 pg/mL
Sample Type: Serum, plasma, tissue homogenates, cell lysates and other biological fluids
Assay Lenght: 3.5h
Research Area: Enzyme & Kinase;Tumor immunity;Immune molecule;Neuro science
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to A Disintegrin And Metalloprotease 8 (ADAM8) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to A Disintegrin And Metalloprotease 8 (ADAM8) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain A Disintegrin And Metalloprotease 8 (ADAM8), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of A Disintegrin And Metalloprotease 8 (ADAM8) in the samples is then determined by comparing the OD of the samples to the standard curve.
