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Product Description
Human APOC3 (Apolipoprotein C3) ELISA Kit | ELK2244 | ELK Biotechnology
Alternative Names: Apo-C3; APOCIII; APOC-III
Species: Human
Assay Type: Sandwich
Sensitivity: 40.65 pg/mL
Standard: 6000 pg/mL
Detection Range: 93.75-6000 pg/mL
Sample Type: Serum, plasma and other biological fluids
Assay Lenght: 3.5h
Research Area: Metabolic pathway;Endocrinology;Cardiovascular biology;Hepatology;Nutrition metabolism
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Apolipoprotein C3 (APOC3) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Apolipoprotein C3 (APOC3) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Apolipoprotein C3 (APOC3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Apolipoprotein C3 (APOC3) in the samples is then determined by comparing the OD of the samples to the standard curve.
