Euro
USD
British Pound
NULL
Product Description
Human CADM1 (Cell Adhesion Molecule 1) ELISA Kit | ELK3689 | ELK Biotechnology
Alternative Names: BL2; IGSF4; IGSF4A; NECL2; RA175; ST17; SYNCAM; TSLC1; sTSLC-1; synCAM1; Tumor Suppressor In Lung Cancer 1; Immunoglobulin Superfamily, Member 4; Nectin-Like 2
Species: Human
Assay Type: Sandwich
Sensitivity: 0.071 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: Serum, plasma, tissue homogenates, cell lysates and other biological fluids.
Assay Lenght: 3.5h
Research Area: CD & Adhesion molecule
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cell Adhesion Molecule 1 (CADM1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cell Adhesion Molecule 1 (CADM1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cell Adhesion Molecule 1 (CADM1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cell Adhesion Molecule 1 (CADM1) in the samples is then determined by comparing the OD of the samples to the standard curve.
