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Product Description
Human CD276 (Cluster Of Differentiation 276) ELISA Kit | ELK3533 | ELK Biotechnology
Alternative Names: B7-H3; B7H3; CD276 Molecule; B7 homolog 3; Costimulatory molecule
Species: Human
Assay Type: Sandwich
Sensitivity: 0.118 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates and other biological fluids
Assay Lenght: 3.5h
Research Area: CD & Adhesion molecule;Tumor immunity;Autoimmunity
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cluster Of Differentiation 276 (CD276) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cluster Of Differentiation 276 (CD276) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cluster Of Differentiation 276 (CD276), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cluster Of Differentiation 276 (CD276) in the samples is then determined by comparing the OD of the samples to the standard curve.
