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Product Description
Human CLU (Clusterin) ELISA Kit | ELK1079 | ELK Biotechnology
Alternative Names: CLU; TRPM2; KUB1; CLI; APOJ; SGP2; AAG4; APO-J; SP-40; Apolipoprotein J; Complement Lysis Inhibitor; 40, Sulfated Glycoprotein 2; Testosterone-Repressed Prostate Message 2
Species: Human
Assay Type: Sandwich
Sensitivity: 5.1 ng/mL
Standard: 800 ng/mL
Detection Range: 12.5-800 ng/mL
Sample Type: serum, plasma, urine, saliva, tissue homogenates, cerebrospinal fluid, cell culture supernates and other biological fluids
Assay Lenght: 3.5h
Research Area: Apoptosis;Tumor immunity;Infection immunity
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Clusterin (CLU) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Clusterin (CLU) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Clusterin (CLU), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Clusterin (CLU) in the samples is then determined by comparing the OD of the samples to the standard curve.
