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Product Description
Human COL6a3 (Collagen Type VI Alpha 3) ELISA Kit | ELK3613 | ELK Biotechnology
Alternative Names: COL6-A3; Collagen Alpha-3 (VI) chain
Species: Human
Assay Type: Sandwich
Sensitivity: 50 pg/mL
Standard: 2000 pg/mL
Detection Range: 31.25-2000 pg/mL
Sample Type: serum, plasma, tissue homogenates and other biological fluids
Assay Lenght: 3.5h
Research Area: Metabolic pathway;Developmental science;Nutrition metabolism;Bone metabolism
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Collagen Type VI Alpha 3 (COL6a3) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Collagen Type VI Alpha 3 (COL6a3) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Collagen Type VI Alpha 3 (COL6a3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Collagen Type VI Alpha 3 (COL6a3) in the samples is then determined by comparing the OD of the samples to the standard curve.
