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Product Description
Human EGFR (Epidermal Growth Factor Receptor) ELISA Kit | ELK2110 | ELK Biotechnology
Alternative Names: ErbB-1; ErbB1; ER1; ERB-B1; MENA; HER1; Erythroblastic Leukemia Viral (v-Erb-B) oncogene Homolog, Avian; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1
Species: Human
Assay Type: Sandwich
Sensitivity: 1.22 ng/mL
Standard: 200 ng/mL
Detection Range: 3.13-200 ng/mL
Sample Type: serum, plasma, tissue homogenates and other biological fluids
Assay Lenght: 3.5h
Research Area: CD & Adhesion molecule;Tumor immunity
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Epidermal Growth Factor Receptor (EGFR) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Epidermal Growth Factor Receptor (EGFR) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Epidermal Growth Factor Receptor (EGFR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Epidermal Growth Factor Receptor (EGFR) in the samples is then determined by comparing the OD of the samples to the standard curve.
