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Product Description
Human EGFR2 (Epidermal Growth Factor Receptor 2) ELISA Kit | ELK2233 | ELK Biotechnology
Alternative Names: CD340; ErbB2; HER-2/neu; HER2; NEU; MLN 19; NGL; TKR1; p185; V-Erb-B2 Erythroblastic Leukemia Viral Oncogene Homolog 2; Neuro/Glioblastoma Derived Oncogene Homolog
Species: Human
Assay Type: Sandwich
Sensitivity: 0.229 ng/mL
Standard: 40 ng/mL
Detection Range: 0.63-40 ng/mL
Sample Type: serum, plasma, tissue homogenates and other biological fluids
Assay Lenght: 3.5h
Research Area: Signal transduction;Tumor immunity;Reproductive science;Hepatology
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Epidermal Growth Factor Receptor 2 (EGFR2) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Epidermal Growth Factor Receptor 2 (EGFR2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Epidermal Growth Factor Receptor 2 (EGFR2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Epidermal Growth Factor Receptor 2 (EGFR2) in the samples is then determined by comparing the OD of the samples to the standard curve.
