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Product Description
Human F5 (Coagulation Factor V) ELISA Kit | ELK1765 | ELK Biotechnology
Alternative Names: FV; FVL; PCCF; Proaccelerin; Labile Factor; Factor V Leiden; Accelerator Globulin; Activated protein C cofactor; Proaccelerin, labile factor
Species: Human
Assay Type: Sandwich
Sensitivity: 61 pg/mL
Standard: 8000 pg/mL
Detection Range: 125-8000 pg/mL
Sample Type: plasma
Assay Lenght: 3.5h
Research Area: Enzyme & Kinase;Metabolic pathway;Hematology
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Coagulation Factor V (F5) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Coagulation Factor V (F5) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Coagulation Factor V (F5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Coagulation Factor V (F5) in the samples is then determined by comparing the OD of the samples to the standard curve.
