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Product Description
Human FOLR1 (Folate Receptor 1, Adult) ELISA Kit | ELK3046 | ELK Biotechnology
Alternative Names: FBP; FOLR; FR-alpha; MOv18; Adult folate-binding protein; KB cells FBP; Ovarian tumor-associated antigen MOv18
Species: Human
Assay Type: Sandwich
Sensitivity: 5.8 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: Serum, plasma and other biological fluids.
Assay Lenght: 3.5h
Research Area: CD & Adhesion molecule
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Folate Receptor 1, Adult (FOLR1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Folate Receptor 1, Adult (FOLR1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Folate Receptor 1, Adult (FOLR1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Folate Receptor 1, Adult (FOLR1) in the samples is then determined by comparing the OD of the samples to the standard curve.
