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Product Description
Human GP5 (Glycoprotein V, Platelet) ELISA Kit | ELK3636 | ELK Biotechnology
Alternative Names: CD42-D; CD42d
Species: Human
Assay Type: Sandwich
Sensitivity: 0.122 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: Plasma
Assay Lenght: 3.5h
Research Area: CD & Adhesion molecule;Infection immunity;Hematology
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glycoprotein V, Platelet (GP5) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Glycoprotein V, Platelet (GP5) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glycoprotein V, Platelet (GP5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glycoprotein V, Platelet (GP5) in the samples is then determined by comparing the OD of the samples to the standard curve.
