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Product Description
Human IL6R (Interleukin 6 Receptor) ELISA Kit | ELK2247 | ELK Biotechnology
Alternative Names: IL-6R;CD126; gp80; IL6R; IL-6R-1; IL-6R-Alpha; IL6RA; IL-6 receptor subunit alpha; Membrane glycoprotein 80
Species: Human
Assay Type: Sandwich
Sensitivity: 0.83 ng/mL
Standard: 120 ng/mL
Detection Range: 1.88-120 ng/mL
Sample Type: Serum, plasma, tissue homogenates, urine and other biological fluids
Assay Lenght: 3.5h
Research Area: Signal transduction;CD & Adhesion molecule;Infection immunity
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 6 Receptor (IL6R) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Interleukin 6 Receptor (IL6R) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 6 Receptor (IL6R), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 6 Receptor (IL6R) in the samples is then determined by comparing the OD of the samples to the standard curve.
