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Product Description
Human KLK3 (Kallikrein 3) ELISA Kit | ELK1197 | ELK Biotechnology
Alternative Names: APS; KLK2A1; PSA; HK3; KLKB1; Seminin; Prostate Specific Antigen; Kallikrein-Related Peptidase 3; Semenogelase, g-Seminoprotein; P-30 Antigen; Plasma kallikrein
Species: Human
Assay Type: Sandwich
Sensitivity: 0.08 ng/mL
Standard: 12.5 ng/mL
Detection Range: 0.2-12.5 ng/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Lenght: 3.5h
Research Area: Tumor immunity;Reproductive science
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Kallikrein 3 (KLK3) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Kallikrein 3 (KLK3) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Kallikrein 3 (KLK3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Kallikrein 3 (KLK3) in the samples is then determined by comparing the OD of the samples to the standard curve.
