Euro
USD
British Pound
NULL
Product Description
Human LIPE (Lipase, Hormone Sensitive) ELISA Kit | ELK1837 | ELK Biotechnology
Alternative Names: HSL; LHS; Hormone Sensitive Lipase
Species: Human
Assay Type: Sandwich
Sensitivity: 0.065 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: serum, plasma and other biological fluids
Assay Lenght: 3.5h
Research Area: Metabolic pathway
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lipase, Hormone Sensitive (LIPE) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Lipase, Hormone Sensitive (LIPE) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lipase, Hormone Sensitive (LIPE), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Lipase, Hormone Sensitive (LIPE) in the samples is then determined by comparing the OD of the samples to the standard curve.
