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Product Description
Human Maspin (Mammary Serine Protease Inhibitor) ELISA Kit | ELK3527 | ELK Biotechnology
Alternative Names: SERPINB5; PI5; Protease Inhibitor 5; Serpin Peptidase Inhibitor Clade B Member 5
Species: Human
Assay Type: Sandwich
Sensitivity: 0.267 ng/mL
Standard: 40 ng/mL
Detection Range: 0.63-40 ng/mL
Sample Type: Serum, plasma, tissue homogenates and other biological fluids.
Assay Lenght: 3.5h
Research Area: Tumor immunity
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mammary Serine Protease Inhibitor (Maspin) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mammary Serine Protease Inhibitor (Maspin) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mammary Serine Protease Inhibitor (Maspin), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mammary Serine Protease Inhibitor (Maspin) in the samples is then determined by comparing the OD of the samples to the standard curve.
