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Product Description
Human MOG (Myelin Oligodendrocyte Glycoprotein) ELISA Kit | ELK2364 | ELK Biotechnology
Alternative Names: BTN6 ; BTNL11 ; MGC26137 ; MOG alpha 5 ; MOG alpha 6 ; MOG AluA ; MOG AluB ; MOG ; MOG Ig AluB ; MOGIG2 ;
Species: Human
Assay Type: Sandwich
Sensitivity: 0.054 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Lenght: 3.5h
Research Area: Infection immunity;Neuro science;Genetic science;Autoimmunity
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Myelin Oligodendrocyte Glycoprotein (MOG) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Myelin Oligodendrocyte Glycoprotein (MOG) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Myelin Oligodendrocyte Glycoprotein (MOG), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Myelin Oligodendrocyte Glycoprotein (MOG) in the samples is then determined by comparing the OD of the samples to the standard curve.
