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Product Description
Human MPIF1 (Myeloid Progenitor Inhibitory Factor 1) ELISA Kit | ELK2475 | ELK Biotechnology
Alternative Names: CCL23; SCYA23; Ckb-8; MIP-3; CKb8; Chemokine (C-C-Motif) ligand 23; Small Inducible Cytokine Subfamily A (Cys-Cys) Member 23; Macrophage inflammatory protein 3
Species: Human
Assay Type: Sandwich
Sensitivity: 0.47 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: Serum, plasma and other biological fluids.
Assay Lenght: 3.5h
Research Area: Cytokine;Tumor immunity;Infection immunity
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Myeloid Progenitor Inhibitory Factor 1 (MPIF1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Myeloid Progenitor Inhibitory Factor 1 (MPIF1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Myeloid Progenitor Inhibitory Factor 1 (MPIF1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Myeloid Progenitor Inhibitory Factor 1 (MPIF1) in the samples is then determined by comparing the OD of the samples to the standard curve.
