Product Description
Human MUC7 (Mucin 7, Secreted) ELISA Kit | ELK2203 | ELK Biotechnology
Alternative Names: MG2; Mucin 7, Salivary; Apo-MG2; Salivary mucin-7
Species: Human
Assay Type: Sandwich
Sensitivity: 0.122 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: serum, plasma, saliva, tissue homogenates and other biological fluids
Assay Lenght: 3.5h
Research Area: Tumor immunity;Infection immunity
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mucin 7, Secreted (MUC7) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mucin 7, Secreted (MUC7) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mucin 7, Secreted (MUC7), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mucin 7, Secreted (MUC7) in the samples is then determined by comparing the OD of the samples to the standard curve.