Product Description
Human PDGFB (Platelet Derived Growth Factor Subunit B) ELISA Kit | ELK1856 | ELK Biotechnology
Alternative Names: PDGF-B; PDGF2; SIS; SSV; C-sis; Simian Sarcoma Viral (v-sis) Oncogene Homolog; Platelet Derived Growth Factor Beta Polypeptide; Proto-oncogene c-Sis; Becaplermin
Species: Human
Assay Type: Sandwich
Sensitivity: 6.3 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Assay Lenght: 3.5h
Research Area: Cytokine
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Platelet Derived Growth Factor Subunit B (PDGFB) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Platelet Derived Growth Factor Subunit B (PDGFB) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Platelet Derived Growth Factor Subunit B (PDGFB), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Platelet Derived Growth Factor Subunit B (PDGFB) in the samples is then determined by comparing the OD of the samples to the standard curve.