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Product Description
Human PIIICP (Procollagen III C-Terminal Propeptide) ELISA Kit | ELK2126 | ELK Biotechnology
Alternative Names: P3CP; C-Propeptide Of Type III Procollagen; Procollagen III Carboxy Terminal Propeptide
Species: Human
Assay Type: Sandwich
Sensitivity: 6.2 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: serum, plasma, tissue homogenates and other biological fluids
Assay Lenght: 3.5h
Research Area: Metabolic pathway;Bone metabolism
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Procollagen III C-Terminal Propeptide (PIIICP) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Procollagen III C-Terminal Propeptide (PIIICP) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Procollagen III C-Terminal Propeptide (PIIICP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Procollagen III C-Terminal Propeptide (PIIICP) in the samples is then determined by comparing the OD of the samples to the standard curve.
