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Product Description
Human PKM2 (Pyruvate Kinase, Muscle) ELISA Kit | ELK1514 | ELK Biotechnology
Alternative Names: M2PK; PKM; M2-PK; PKM1; CTHBP; OIP3; PK3; PKM; TCB; THBP1; Pyruvate kinase muscle isozyme; Thyroid hormone-binding protein 1; Cytosolic thyroid hormone-binding protein
Species: Human
Assay Type: Sandwich
Sensitivity: 30 pg/mL
Standard: 5000 pg/mL
Detection Range: 78.13-5000 pg/mL
Sample Type: serum, plasma, tissue homogenates and other biological fluids
Assay Lenght: 3.5h
Research Area: Enzyme & Kinase;Tumor immunity
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pyruvate Kinase, Muscle (PKM2) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pyruvate Kinase, Muscle (PKM2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pyruvate Kinase, Muscle (PKM2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pyruvate Kinase, Muscle (PKM2) in the samples is then determined by comparing the OD of the samples to the standard curve.
