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Product Description
Human PRB1 (Basic Salivary Proline Rich Protein 1) ELISA Kit | ELK3536 | ELK Biotechnology
Alternative Names: PM; PMF; PMS; PRB1L; PRB1M; Ps 1; Ps 2; PRPPRB1; Ps; cP7; Proline-Rich Protein BstNI Subfamily 1
Species: Human
Assay Type: Sandwich
Sensitivity: 0.59 ng/mL
Standard: 100 ng/mL
Detection Range: 1.57-100 ng/mL
Sample Type: saliva and other biological fluids
Assay Lenght: 3.5h
Research Area: Infection immunity
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Basic Salivary Proline Rich Protein 1 (PRB1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Basic Salivary Proline Rich Protein 1 (PRB1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Basic Salivary Proline Rich Protein 1 (PRB1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Basic Salivary Proline Rich Protein 1 (PRB1) in the samples is then determined by comparing the OD of the samples to the standard curve.
