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Product Description
Human PRB2 (Basic Salivary Proline Rich Protein 2) ELISA Kit | ELK3535 | ELK Biotechnology
Alternative Names: PRPPRB1; Ps; cP7; Proline-Rich Protein BstNI Subfamily 2; Con1 glycoprotein; Basic peptide P-F
Species: Human
Assay Type: Sandwich
Sensitivity: 0.52 ng/mL
Standard: 80 ng/mL
Detection Range: 1.25-80 ng/mL
Sample Type: saliva and other biological fluids
Assay Lenght: 3.5h
Research Area: Infection immunity
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Basic Salivary Proline Rich Protein 2 (PRB2) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Basic Salivary Proline Rich Protein 2 (PRB2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Basic Salivary Proline Rich Protein 2 (PRB2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Basic Salivary Proline Rich Protein 2 (PRB2) in the samples is then determined by comparing the OD of the samples to the standard curve.
