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Product Description
Human SLC1A5 (Solute Carrier Family 1, Member 5) ELISA Kit | ELK3497 | ELK Biotechnology
Alternative Names: SLC1A5; AAAT; ASCT2; ATBO; M7V1; M7VS1; R16; RDRC; Neutral Amino Acid Transporter B (0) ; Baboon M7 virus receptor; Sodium-dependent neutral amino acid transporter type 2
Species: Human
Assay Type: Sandwich
Sensitivity: 0.124 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: Tissue homogenates, cell lysates and other biological fluids
Assay Lenght: 3.5h
Research Area: Signal transduction
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Solute Carrier Family 1, Member 5 (SLC1A5) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Solute Carrier Family 1, Member 5 (SLC1A5) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Solute Carrier Family 1, Member 5 (SLC1A5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Solute Carrier Family 1, Member 5 (SLC1A5) in the samples is then determined by comparing the OD of the samples to the standard curve.
