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Product Description
Human SPA (Surfactant Associated Protein A) ELISA Kit | ELK1644 | ELK Biotechnology
Alternative Names: PRL; SFTPA; PSAP; PSPA; SFTP; SFTPA1; COLEC4; PSP-A; SFTP1; SFTPA1B; SP-A1; Collectin-4; Alveolar proteinosis protein; 35 kDa pulmonary surfactant-associated protein
Species: Human
Assay Type: Sandwich
Sensitivity: 17.43 pg/mL
Standard: 3000 pg/mL
Detection Range: 46.88-3000 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, lung lavage fluid, cell culture supernates and other biological fluids
Assay Lenght: 3.5h
Research Area: Infection immunity;Pulmonology
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Surfactant Associated Protein A (SPA) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Surfactant Associated Protein A (SPA) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Surfactant Associated Protein A (SPA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Surfactant Associated Protein A (SPA) in the samples is then determined by comparing the OD of the samples to the standard curve.
