Euro
USD
British Pound
NULL
Product Description
Human WISP1 (WNT1 Inducible Signaling Pathway Protein 1) ELISA Kit | ELK3406 | ELK Biotechnology
Alternative Names: CCN4; WISP1c; WISP1i; WISP1tc; CCN family member 4; Wnt-1-induced secreted protein
Species: Human
Assay Type: Sandwich
Sensitivity: 13.3 pg/mL
Standard: 2000 pg/mL
Detection Range: 31.25-2000 pg/mL
Sample Type: serum, plasma, tissue homogenates and other biological fluids
Assay Lenght: 3.5h
Research Area: Signal transduction
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to WNT1 Inducible Signaling Pathway Protein 1 (WISP1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to WNT1 Inducible Signaling Pathway Protein 1 (WISP1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain WNT1 Inducible Signaling Pathway Protein 1 (WISP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of WNT1 Inducible Signaling Pathway Protein 1 (WISP1) in the samples is then determined by comparing the OD of the samples to the standard curve.
