Product Description
Human XRCC6 (X-Ray Repair Cross Complementing 6) ELISA Kit | ELK1932 | ELK Biotechnology
Alternative Names: Ku70; CTC75; CTCBF; G22P1; ML8; TLAA; Lupus Ku Autoantigen Protein P70; ATP-Dependent DNA Helicase 2 Subunit 1; CTC box-binding factor 75 kDa; Thyroid-lupus autoantigen
Species: Human
Assay Type: Sandwich
Sensitivity: 0.124 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: tissue homogenates, cell lysates and other biological fluids
Assay Lenght: 3.5h
Research Area: Signal transduction;Developmental science
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to X-Ray Repair Cross Complementing 6 (XRCC6) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to X-Ray Repair Cross Complementing 6 (XRCC6) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain X-Ray Repair Cross Complementing 6 (XRCC6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of X-Ray Repair Cross Complementing 6 (XRCC6) in the samples is then determined by comparing the OD of the samples to the standard curve.